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Because a compound is characterized by the grouping of atoms and each atom has a characteristic mass, it was at once easy to understand that compounds have a fixed composition by mass.
Moreover, the existence of related families of compounds, which differ in an integral manner in their composition by mass, could immediately be explained by supposing that the various compounds differ in the number of atoms of one element that combine with one atom of a second element.
First, in samples from early foraging trips, the relatively low level of digestion for most samples meant that the composition by mass could be determined directly.
For the calculation of composition by mass for each sample, the unidentifiable component of the sub-sample was assumed to contain the same proportions of prey as the identifiable component, and the sub-sample was assumed to be representative of the entire sample [3].
Fibers containing dexamethasone were fabricated to have a 5.7% composition (by mass) of the drug so that the total quantity implanted would be comparable to doses given as intraperitoneal injections to rodents.
Since Elba was substantially enriched in the 1.0 M KCl fraction from WT beads (WE1), while there was little in the corresponding fraction from mutant beads (WE2) (not shown), we analyzed the protein composition by mass spectrometry.
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Now that proteomic analyses are possible, where cell lysates or similar samples are subjected to cleavage by a peptidase, peptides isolated, composition determined by mass spectroscopy, and possible source protein(s) determined from the composition (19), the amount of data is set to rise exponentially.
The protein spot is then excised from the gel and its composition determined by mass spectrometry [ 43, 44].
A similar approach has been used to compare gene expression data from mouse embryoid bodies versus embryonic stem cells, determined by quantitative real-time PCR (qRT-PCR) with the sphingolipid composition determined by mass spectrometry.
Due to the low sequence similarity of human and the characterized yeast Mon1-Ccz1 complex, the human protein complex was first isolated and the subunit composition confirmed by mass spectrometry.
The deposition yield and coating composition were determined by mass and thermogravimetric analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com