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Documentation of the Jurassic specimens followed the principles of fluorescence composite imaging and macro-fluorescence imaging (see [ 26],[ 27]).
Our study, like some other recent reinvestigations [ 5, 25, 37] took advantage of new investigation techniques (polarized light, composite imaging) and the availability of more taxa for comparison.
Overview images, in particular, were taken as several photographs in different x- and y-positions and then stitched together with Adobe Photoshop CS3 or Microsoft Image Composite Editor (for details on composite imaging see [ 15]).
The overview images of the other specimens (Al. helonomus, Hyperia sp., Pa .lacustris, and Neo. semissis; see Table 4) were documented with fluorescence microscopy and composite imaging [ 35, 69].
Small specimens were documented using the UV-fluorescence composite imaging method [ 25] (wavelength ca. 358 nm) or, depending on the autofluorescence capabilities of the specimens, with orange-green fluorescence (wavelength ca. 546 nm) [ 17].
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The composite image validates that pvOCT identifies most of perfusion structures compared with the average intensity imaging where vessels can be classified by relatively bright structures.
CT acquires imaging data rapidly within one breath-hold, whilst PET takes many minutes to accumulate a composite image during which time the patient is breathing.
This composite image can be thought of as a map of the best estimation of where the fluorophores are located during imaging.
Composite image including, concentric from the outside: Micrasterias rotata, Micrasterias sp., M. furcata, M. americana, 2x M. truncata, Euastrum sp. and Cosmarium sp. Technique: Confocal imaging, 400x.
Composite functional/anatomical imaging (SPECT-CT) has the potential to enhance topographic orientation and diagnostic sensitivity of sentinel lymph node imaging, but has not yet been applied in the head and neck region.
The inter-observer agreement with composite UWF imaging (k = 0.65) was substantial, while inter-observer agreement with Zeiss (k = 0.47) was moderate.
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