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The total aligned reads and total gene count were iteratively increased until the major components of variation were not associated with alignment depth or gene count.
Components of variation due to measurement system and manufacturing process must be estimated and special causes of variation should be reduced whenever possible.
Option value corresponds not just to unknown future values of known species, but also to the unknown values of unknown species (or other components of variation).
Unless the non-genetic components of variation also happen to be concentrated in the direction of this particular line or plane, the two components of variation will therefore be different.
Even considering only time series data within subject, inter-regional correlations depend on which components of variation are retained.
We applied PCA separately to each center to see if the same components of variation could be identified.
Two clustering approaches were used to determine components of variation in the data in this study as follows.
A more stringent evaluation was provided by a SVD to check whether components of variation were correlated with unwanted factors.
After inter-array normalisation using either method (d) or (e), the largest components of variation were associated with phenotypic factors such as age and cancer (Figure S1c).
TIGR MeV (Multiple Experimental Viewer) software [59] was utilized to create clustergrams as well as principal component analysis plots of the first three components of variation.
The statistical significance of the components of variation inferred using SVD was evaluated against the null distribution obtained by considering random matrices.
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