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The various components of this cholesterol flux can be viewed as efflux, influx and net flux.
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Visualization of this pathway revealed that the genes identified were components of the cholesterol biosynthetic pathway.
Figure 4F shows a heatmap of these Srebp2 target genes [ 63] and impressively demonstrates reduction of transcripts for nearly all components of the cholesterol biosynthesis and uptake pathway in the investigated tissues.
Rather, it suggests that the overall process is likely complex and that paraoxon may affect multiple components of the cholesterol efflux machinery, including several other candidates that catalyze cholesteryl ester hydrolysis that are known to exist.
Notably, P. chabaudi PcEMA1 proteins could constitute a second component of this proposed cholesterol import machinery.
Together, these data led to the hypothesis that StarD3 might be an important component of the cholesterol homeostasis mechanisms sustaining effective insulin release in β-cells.
The third enzyme, ERG7p, converts squalene epoxide into lanosterol, the first cyclic component of the cholesterol biosynthesis cascade (i.e. the first sterol).
Lanolin may be the active anti-ON component of the cholesterol diet.
This is in line with the observation that phospholipid content of HDL, a critical component of the cholesterol acceptor capability of mature HDL, is not altered in elderly subjects [13,51].
There is also evidence that pitavastatin may increase HDL-C by increasing expression of ApoAI and ATP-binding cassette transporter A1, key components of reverse cholesterol transport [ 84– 86].
If we take the gene network approach, a recent version involved a boolean network model that assigned 33 different components of intracellular cholesterol homeostasis a boolean value.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com