Sentence examples for components of the mobile phase from inspiring English sources

The parameters involved were: the pH of the phosphate buffer used in the mobile phase, the ratio between the components of the mobile phase, pH of the derivatization reaction mixture, and column temperature.

The condition, however, that the composition of all components of the mobile phase must total 100% presents a problem when trying to mathematically represent ranges of each mobile phase constituent of a ternary or quaternary system.

The addition of a small amount of acetone to both components of the mobile phase resulted in narrower peaks for acesulfame-K, saccharin, aspartame, sucralose, cyclamate, alitame, neohesperidin DC and neotame.

The chiral column is very sensitive to any changes in mobile-phase composition and, to ensure reproducible retention times, is it advisable to mix the individual solvent components of the mobile phase via the inlet method of the HPLC.

The aspects that separate the 2 techniques stem from the use of carbon dioxide (CO2) as the main component of the mobile phase in SFC.

A complicated buffer is a component of the mobile phase for the HPLC method, which includes monobasic potassium phosphate (6.8 g), 1-pentane sulphonic acid sodium salt monohydrate (1.28 g) and triethylamine (3 mL), and the pH is adjusted to 3.0 with orthophosphoric acid (Rele and Warkar 2011).

The absence of non-volatile components in the mobile phase allows running the method with evaporative light scattering and MS-detectors.

As previous reported methods may not be applied for routine analysis due to the use of the triethylamine component in the mobile phase, this novel RP-HPLC method might be applied for assays, dissolution studies, bio-equivalence studies as well as routine analysis of OLO in pharmaceutical industries.

The consequences of accounting for the adsorption of the mobile phase components themselves are investigated for the experimentally common situation of a linear inlet gradient.

Hence, the ratio of the mobile phase components n-Hexane/alcohol/diethylamine was fixed at 85:15:0.01 (v/v/v) and the effect of different alcohols on selectivity was studied (Fig. 3).

A Box-Behnken experimental design was used to study different experimental variables of the method, namely the ratio of the mobile phase components and the gradient time, and their influence in responses such as the retention factor, tailing factor, and theoretical plates for dapivirine and the IS, as well as the peak resolution between both compounds.