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Assays to evaluate cell-matrix interactions effects on components of repair were employed.
Histone H2A.X localizes to DNA double-strand breaks, where it recruits components of repair machinery.
Consistently, the majority of the NUDIX proteins appear to be "house cleaning" enzymes rather than bona fide components of repair systems [ 74].
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Also mobilization and release of the rotator cuff tendon, known to be important component of repair of retracted rotator cuff tears was not assessed in these cadaveric shoulders.
Analysis of the repair kinetics showed that almost all damage repaired under conditions of stimulated glycolysis was handled by the fast component of repair (~93%) as compared to the un-stimulated cells (~45%).
DNA-PKcs, an enzyme involved in DNA repair, was chronically activated but failed to promote DNA repair due to the deficiency of essential components of the repair complex, specifically the DNA-binding proteins Ku70 and Ku80.
In order to determine whether SpFan1 could be involved in other pathways of DNA repair, we performed in vivo survival assays to assign genetic interactions between SpFan1 and known components of characterized repair pathways involved in the ICL response.
MutL homologs (MLH/PMS) are key components of mismatch repair (MMR).
Remarkably, 10 of the 15 genes are conserved in other bacteria and are well-characterized components of DNA repair pathways.
Finally, USP1 deubiquitinates and inactivates two components of DNA repair mechanisms: FANCD2 (a component of the Fanconi Anemia pathway) [4], [69] and PCNA [70].
This shift could further be promoted by aging-associated defects in components of DNA repair pathways.
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