To test this hypothesis, four site-specific inhibitors of P-ETC components were administered and their effect on electrogenic activity was recorded using two individual cultures, Nostoc and Lyngbya.
25 The two main components, the functional and disability components, were administered at baseline and 12 months' follow-up.
In addition to examples in which each component caused measurable effects, examples were cited in which combined exposures caused measurable effects that were not observed when the components were administered individually at the same doses.
Because it was not viable to produce a custom supplement for this study, commercially available Australian calcium supplements, which are typically made from overseas components, were administered to the subjects.
In all these studies, a joint effect of 50% was observed when the mixture components were administered at concentrations equivalent to 2.4 9.6% of their individual median effective concentration (EC50).
When the components were administered in combination at the highest concentrations used, they markedly and significantly reduced ROS production in NCTC 2544 cells, both in basal conditions (93% reduction, P < 0.027), as well as with H2O2 (57% reduction, P < 0.0099).
The survey's components were administered in homes and in mobile examination centres, and also provided national reference data for biological markers, anthropometric measures, as well as demographic and socioeconomic status data.
