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The relation between the size of the small event, size of the subfault, and frequency component to be analyzed is as follows: In this formulation, a sufficiently small event that can be regarded as a point source is used (condition 1).
It should be added that difference in time observed in Fig. 1 is due to the nature of samples analyzed, where in sample 1, rTat was the only component to be analyzed while in other panels Tat was issued from either the supernatant of infected cells or from cell extracts after adding the infected/isolated supernatant.
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It requires the use of sufficiently small subfaults, depending on the frequency components to be analyzed.
Typical applications of suboptimal methods are to provide reliable estimates for data reconciliation algorithm initialization, support the selection of components to be analyzed, and to detect faults prior mass balancing calculations.
The extracellular protein collagen I, matrix metalloproteinases MMP-1 and MMP-3, and the glycosaminoglycan hyaluronic acid were chosen as representative ECM components to be analyzed in this study.
The proceedings and findings of our adaptation study imply that context, construct, and IT components need to be analyzed before doing adaptations.
Second, there is a question of how many principal components are to be analyzed.
Further, the signal to noise ratio has several components that have to be analyzed.
Conversely, targeted MS approaches, such as selected reaction monitoring (SRM -based methodSRM -basedd in [ 19]), require a priori knowledge of the protein comethodss of intereviewedbe analyzed, and hence can only be used to measure pre-selected proteins.
Although this approach is widely used and allows sensitive detection of protein complex components, each band has to be analyzed separately and some protein complex components can be missed due to gel background: In the example shown in Figure 3B, left, protein Y is specific to FLAG-p160 complex, but it may not be identified because of a co-migrating background band.
Artifacts (over- or under-damping) should be carefully identified and eliminated, especially when systolic-diastolic components and waveform have to be analyzed.
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