The two kinds of component proteins of the artificial organelle produced ATP, and the resulting ATP drove protein synthesis.
We also confirmed the same amount of Fo component proteins were photosynthesized in both samples (Supplementary Fig. 24C).
To test whether our artificial photosynthetic cell system can synthesize the component proteins of its own artificial organelle, we tried to photosynthesize bR, as well as FoF1.
We next synthesized only three component proteins of Fo, a-, b- and c-subunits, in the presence of purified F1 and bR-PLs.
Domain fusion analysis has been proposed recently to infer the functional association of the component proteins.
First, the microtubule system in Giardia appears to contain a complete array of component proteins, including all tubulin isoforms, and many kinesins and dyneins.
They seem to act as crucibles to speed up reactions between their component proteins in some cases, and as a means of keeping reactants away from one another in others.
Following this, we photosynthesized de novo Fo component proteins in the presence of bRFoF1-PLs (0.3 µM FoF1 and 140 µM bR) and purified F1 in the PURE system suited for mRNA start (Supplementary Table 2 and Supplementary Fig. 1F).
This material has a remarkable combination of flexibility and durability: Elastin is one of the body's most long-lasting component proteins, with an average survival time comparable to a human lifespan.
Progress towards a deeper understanding of cellular biochemical networks demands the development of methods to both identify and validate component proteins of these networks.
SYCP2 and SYCP3 are the main component proteins of the LEs (Hosoya et al., 2012; Yang et al., 2006).
