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In this gradient design strategy, the slope is adjusted after the elution of each individual component of the sample, letting the retention properties of the different analytes auto-guide the course of the gradient profile.
The HPLC-DAD chromatogram was not easy to interpret, but HPLC-ESI-Q-ToF allowed the identification of the main component of the sample.
Its presence could represent an impurity in the column [39], but as it appears in different quantities throughout the samples, it could also be a component of the sample or a by-product of the aging process.
Note that IL, in fact, is also influenced by the difference in mass absorption at the Kα and Kβ energies; if the fluorescing element is a trace or minor component of the sample, the change in mass absorption as a function of concentration will be negligible.
Another component of the sample is seaweed extract from A. nodosum, Fucus spp. and Laminaria spp., and the four main carbohydrates in these species alginic acid, fucoidan, laminarin and mannitol produce characteristic pyrolysis products which can be used to predict their presence [12, 43, 51].
This may be a result of αS-EGFP fibrils remaining suspended in solution, or the fibril fraction may be a minor component of the sample.
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The lignin component of the samples was characterized by CuO oxidation.
The total organic component of the samples examined through LOI is relatively high (all > 2%), with those of the overlying Holocene deposits higher than those of the underlying Malan loess by about 0.2%.
"Figuring out which component of the samples Elvis recognizes in the pregnant bears may allow us to work backwards and finally identify the polar bear pregnancy factor, once and for all," said Dr. Curry.
Furthermore, most of these series were published in endemic regions and ALM and MCM melanoma are usually not a substantial component of the samples studied.
Finally, the collected organic component of the samples were evaporated to dryness under nitrogen flow using TurboVap-II (Caliper Life Sciences, USA) and re-suspended in methanol for chromatographic analysis.
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