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Dr. Wang and Dr. Michael H. Roehrl of Harvard believe the target is a type of carbohydrate known as glycosaminoglycans, a major component of cartilage, joint fluids, connective tissue and skin.
Chondroitin sulfate is an essential component of cartilage and is present also in synovial fluid.
Chondroitin sulfate (CS) is an acidic glycosaminoglycan (GAG), which constitutes an important component of cartilage, blood vessel walls, skin, tendons, and other connective tissues [21].
Hyaluronic acid (or hyaluronan), a natural component of cartilage, is a linear polysaccharide comprising repeating disaccharide units of D-glucoronic acid and N-acetyl-D-glucosamine.
Chondrocytes represent the sole cellular component of cartilage, and regulate its fate due to their ability to synthesize matrix-degrading enzymes and matrix proteins such as collagens and proteoglycans, which are responsible for the tensile strength and compressive resistance, respectively, of cartilage to mechanical loading.
Proteoglycan content, predominantly aggrecan, was determined because it forms a major structural component of cartilage [ 24].
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This review will describe the nanodrugs targeted to specific components of cartilage matrix to generate drug reservoirs within the cartilage.
FT-IRIS permits evaluation of early-stage matrix changes in the primary components of cartilage, collagen and proteoglycan on histological sections at a spatial resolution of ∼6.25 μm.
Differentiation of B10 cells into chondroblasts was determined by staining the cell pellets with Alcian blue which identifies proteoglycan extracellular matrix, specific components of cartilage tissues (Figures 2B D).
In summary, the ChIP-on-chip experiment using chondrocyte chromatin has identified SOX9 interaction sites in a number of genes for components of cartilage as well as for transcription factors and signaling molecules that participate in the regulation of the chondrocyte program.
Chondrocytes are the only cellular components of cartilage.
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