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Table 1 summarizes the final docking score of each component bound to the SCF-binding site of the c-kit receptor protein.
In nuclear extracts prepared from human keratinocytes, JunB was the predominant Jun component bound to the DNA probe containing the same cis element we tested here [ 14].
However, under saturating conditions (≥ 100% BMAX; Figure 9A, B, middle panels), which represents the curcuminoid concentrations employed in the serum competition experiments, the primary component bound to cells incubated with curcuminoids solubilized in serum was CUR (61%), whereas BDMC (48%) predominated in cells incubated with curcuminoids solubilized in HP-γ-CD.
The sequestering function of scaffolds is implemented by expanding the system to include two different states of the "shared" component: bound to the scaffold (denoted x 1 N, for aNchored to the scaffold), and free in the cytosol (denoted x 1 C ).
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The time constant of the redox cycle of the RC is determined by the redox components bound to it.
Here, we combine the reconstructions of each of the individual components bound to actin into a single, unique, self-consistent model of the microvillar and brush border cytoskeleton.
Alternatively, there might be a rapid exchange of protein components bound to the oligonucleotide.
This confers the possibility to create asymmetric assemblies with different components bound to only one side of a membrane or to embed transmembrane structures in distinct orientations.
RAW 264.7 macrophages were denatured by addition of 10 mL hydrochloric acid and phosphate-buffered saline (PBS; pH 4.0) to liberate components bound to cells whithout damaging the physiological state of cells.
Components bound to the beads were eluted by boiling in SDS sample buffer, and then separated on a SDS-PAGE gel and immunoblotted with anti-OsPUB15 (Beijing Protein Innovation Co., Ltd., China) or anti-GST antibody (Beijing Protein Innovation Co., Ltd., China).
Furthermore, the targeting and functional element components bind to each other and successfully form two-component immunogens.
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