The basic steps include reverse transcription of messenger RNA (mRNA) to complimentary DNA (cDNA) followed by cDNA amplification via PCR [9].
The standardised solutions of total cellular mRNA were reverse transcribed to synthesise complimentary DNA (cDNA) using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA).
For analysis, 400 ng of total RNA was subjected to complimentary DNA (cDNA) synthesis using the iScript cDNA synthesis kit Bio-Rad Laboratoriess, Hercules CA) in a volume of 20 µl.
The standardised solutions of total cellular mRNA were reverse transcribed to synthesise complimentary DNA (cDNA) using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA).
Based on the sequence information of the partial complimentary DNA (cDNA) of Foxl2, rapid amplification of cDNA ends (RACE) procedures were performed in order to isolate the 5' and 3' ends of the cDNA (SMART cDNA library construction Kit; Takara, Shiga, Japan).
The extracted tcRNA was treated with DNase (Ambion, UK) and then reverse transcribed to complimentary DNA (cDNA) using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, USA).
Extracted RNA from two plantlets of a species were pooled prior to complimentary DNA (cDNA) synthesis, and 1 mg of this pool was used as template for cDNA synthesis using the SuperSmart PCR cDNA Synthesis kit (Clontech, Mountain View, CA).
The aptamer recognizes and binds to OTA, thus leading to the formation of duplex DNA between the complimentary DNA (cDNA) strand and the signal probe.
To validate a rat model of endometriosis using complimentary DNA (cDNA) microarrays by identifying common gene expression patterns between experimental and natural disease.
Complimentary DNA (cDNA) was synthesized and diluted according the methods described previously [47].
