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Advances in analytical methods and increased resolution of molecular markers are helping scientists to better understand this complicated vector system (Beidler et al. 2003; Barnes et al. 2005; della Torre et al. 2005).
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In contrast with human surveillance, which generally focuses on disease incidence and/or prevalence in a particular population, unique aspects of vector transmission complicate vector surveillance approaches.
We directly injected DNA into the brain because that approach would not be complicated by vector delivery.
The procedure for generating stable cell lines by retroviral vectors or lentiviral vectors is complicated with a stringent specification for the materials and equipments.
Although there is a growing number of applications where transient gene therapy could improve the curative potential of engineered cells, currently available methods (which, like the chronic expression methods described above, are mostly based on viral vectors) are complicated by the time and expense involved in the elaborate protocols required for their implementation20.
After the arrival of the direct waves, the distribution of the slowness vectors became complicated but not random (right panel of Fig. 10b).
However, although preliminary in vitro experiments suggest no obvious cytotoxicity of MoS2-PEG nanosheets, we should still keep in mind that the factors controlling the pharmacokinetics and biodistribution of non-viral vectors were complicated in vivo.
The control of dengue vectors is complicated by a lack of trained personnel and resources and by adopting reactive approaches to dengue control only during epidemics.
The theory of modules, compared to that of vector spaces, is complicated by the presence of ring elements that do not have multiplicative inverses.
Therefore, a practical interpretation of the loadings vectors is often complicated, especially for high-dimensional data.
Alternative approaches based on the use of conventional lentiviral vectors (lentivectors) are complicated by the problem of genomic integration of transgenes and viral DNA elements, potentially precluding their use for clinical applications.
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