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At the same time, the TMD avoids complicated sampling and transferring steps of large-volume water samples.
However, most of the MS methods need complicated sampling interface system.
Although these assays have shown reliable and reproducible results, complicated sampling procedures were required, they frequently involved cell destruction, and the obtained data was acquired at a specific time point (end-points) [19, 20].
The latter is laborious, requires more animals due to the limited blood volume, and is characterized by complicated sampling intervals within a specific time window.
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Fluorimetric, phosphorimetric, and immunosensor methods require complicated sample pre-treatments.
Importantly, it could be applied to complex samples without complicated sample pretreatment and sophisticated instruments.
The whole assay process can be accomplished within 20 min without complicated sample pretreatment.
This proposed sensor displays excellent sensitivity and selectivity, and is applied for detection in complicated samples successfully.
Overall, this study provides a versatile approach for highly efficient fabrication of well-constructed MMIPs for enrichment and determination of target molecules from complicated samples.
PCR-based methods are useful techniques for amplification of small amounts of genetic material but require complicated sample prepurification before analysis.
In order to overcome the limitations of standard mechanical tests delivering merely integral information about complicated samples, experiments were designed for step-wise mechanical testing with concurrent imaging of trabecular and cortical bone.
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