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Although iPCR have been designed to detect many proteins [10 19], it may suffer from important limitations including complicated protocol as well as requirement of special instruments and well-trained laboratory personnel.
Second, the number of subjects (n = 15) is a function of this being a controlled human exposure study involving a complicated protocol.
Actually, the complicated protocol structure makes the analysis intricate and unsuitable for generalization.
Regulators imposed a complicated protocol for cleaning the tower, which they suspected had been contaminated by asbestos and other toxic substances.
Using a more complicated protocol, this method was extended to introduce up to five site-directed mutations, although the complete mutagenesis efficiency decreased to 32% [ 14].
However, a limitation for large-scale analysis is sample preparation (due to complicated protocol, lack of equipment and/or inadequate funding, especially in developing countries) and sample shipment as cooled/frozen specimens.
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If small plates raise a series of questions about etiquette (is it polite to wait until everyone else has snapped up a deep-fried artichoke heart before you charge in for more?), dishes for two come with their own complicated protocols.
However, these powerful techniques require the use of sophisticated instruments and complicated protocols.
Limited energy, computational, and communication resources complicate protocol design within sensor networks and prevent the application of many techniques used within other networks.
This stage would obviously add serious diplomatic challenges to an already complicated scientific protocol.
Unfortunately, this test is very difficult to implement with HFOV and would have slowed and complicated the protocol.
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CEO of Professional Science Editing for Scientists @ prosciediting.com