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Enzymatic sensors possess high selectivity but suffer from limitations such as instability, complicated modification procedures, and critical microenvironmental factors.
Thus, MIP allow for the reconstruction of a vascular map quickly without the need for a complicated modification of multiple parameters.
Comparing with other nanoparticle-based DNA detection assays, our method has the following advantages: (1) no need of complicated modification of GNPs or DNA, (2) no additional DNA probes are required, (3) no need of signal amplification or temperature control.
However, to design a SERS-based biosensor, almost all currently used methods involve the time-consuming and complicated modification of the metallic nanoparticles with the Raman active dye and biorecognition element, which restricts their widespread applications.
This required a more complicated modification.
By adding the ordering information underlying exposure group means in the estimation procedure, improvement of GBS could be possible to a certain extent without any complicated modification even when we have a small number of observations for the exposure in each group.
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The improved cycle stability are competitive or superior to those of many other systems with more complicated modifications.
The dissolution of snowmelt agent Mafilon (MFL) causes complicated modifications in the pore structure of anti-freezing asphalt mixtures.
A complete picture of the genes and molecular mechanisms underlying these traits is often lacking, which complicates targeted modification.
For instance, typical synthetic methods generally need complicated post surface modification to couple with functional groups for hydrophily and biocompatibility [16], which is a two-step synthesis.
Self-assembly is a spontaneous process by which organized structures with particular functions and properties could be obtained without additional complicated processing or modification steps.
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