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These additional kinetic phases introduced data-scatter or atypical baselines that complicated data analysis and curve-fitting (Fig. S3).
This circuit-based approach, however, poses novel problems for the already complicated data analysis in expression microarray studies.
The large volume of data and complicated data analysis pipelines has brought us a significant challenge in both technology development and practical applications.
Arguments against performing dynamic scans are the demanding scanning protocol and the more complicated data analysis.
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Surfactant-induced drainage has been observed to occur for this method in some cases, which can complicate data analysis and impart uncertainty to the measured values.
The T7 system provides greater diversity than the M13 system, is extremely stable, amplifies in a few hours instead of overnight, and can display larger peptides on its protein coat, whereas M13 amplification methods can allow phages with better growth abilities to take over the amplification culture which can decrease diversity with successive rounds of panning, complicating data analysis [30].
This conversion does not appear to complicate data analysis as observed for DSSO cross-linked peptides.
The lack of sample management further complicates data analysis and increases the potential for errors.
At present, NeuCode SILAC-labeled intact protein spectra are incompatible with existing top-down search algorithms, complicating data analysis.
These sequencing-based methodologies present additional challenges as FFPE-derived samples are acknowledged to give rise to sequencing artefacts, complicating data analysis [ 12, 29].
Therefore, the presented study had to be designed as a cross sectional study obtaining morphometric measurements only at a single point in time from each tooth, which inevitably complicates data analysis and interpretation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com