Sentence examples for complicate purification steps from inspiring English sources

The presence of cell vesicles may also complicate purification steps in downstream processing, resulting in a similar contamination problem that exists with VLP production in the baculovirus-insect cell system (similar size of particles, vesicles carrying HA).

rmCRP was expressed in E. coli as a highly insoluble aggregated inclusion body, which complicated purification and characterization analyses.

The expressed product remained within the host cell and necessitates complicated purification methodologies.

This enzymatic process complicates the purification step and increases production costs (Futerman et al.2004).2004

Since GAD is active only at acidic conditions even though pH increases as the reaction proceeds, the conventional practice of using this enzyme involved the use of relatively high concentration of buffers, which might complicate the downstream purification steps.

Alternatively, photoactive protein Z constructs have been made by post-translationally modifying a recombinant protein, but the need for additional reaction and purification steps complicates production and limits the final yield.

However, current methods using hES cells are complicated by the requirements for cocultures with cell lines, use of complex cytokine cocktails and additional purification steps, which limits their use in studies requiring high numbers of homogenous cells.

Current methods using similar approaches with hES cells are complicated by the requirements for cocultures with other cell lines, the use of complex cytokine cocktails and additional purification steps.

Other purification steps are added, including solvents that destroy the lipid shells of viruses like H.I.V. or West Nile.

(A) Purification steps followed by SDS-PAGE.

HPLC chromatograms at different purification steps.