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With increasing complexity of the template pool, this stipulation becomes less likely.
The major handicap for the relevant OVI was the complexity of the template, which needs revision to make it user-friendly.
However, this increases the risk that certain alleles do not hybridize, which again increases with the complexity of the template (number of alleles) because of competition between alleles during the hybridisation reaction.
By pooling a genomic DNA library made from over 9,000 N-ethyl-N-nitrosourea (ENU) mutagenized F1 fish into 32 equal pools of 288 fish, each with a unique Illumina barcode, we reduce the complexity of the template to a level at which we can detect mutations that occur in a single heterozygous fish in the entire library.
Similar(56)
There are several methods which aim to reduce the complexity of the sequencing template, in particular, by reducing the representation of low-information-content repetitive sequences.
The choice of restriction enzyme depends on the complexity of the target templates [ 25].
In view of the complexity of multi-step template preparation and low production, it is imperative to develop simpler template-free methods for the fabrication of magnetic Co nanowires.
In order to investigate the complexity of the mechanistic basis for template selective recognition in these polymeric matrices, the use of a quantum chemical approach has been attempted providing new insights about the mechanisms underlying template recognition, and in particular the crucial role of the crosslinker agent and the solvent used.
TTAS is independent of the content or complexity of template sequences.
This new biomarker relates the kinetics of clot formation to clot outcome in whole blood and allows us to quantify the complexity of the fibrin network microstructure which is believed to be the template for development of the mature clot.
As an approach to recapitulate the complexity of the extracellular matrix, collagen-mimetic proteins offer a modular template to incorporate bioactive and biodegradable moieties into a single construct.
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CEO of Professional Science Editing for Scientists @ prosciediting.com