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Limitations consist of the complexity of the sample design and the lack of longitudinal follow-up of the sample.
Thus, all other peaks on the MS spectrum can be ignored, which greatly reduces the complexity of the sample.
When the complexity of the sample is high, the gradient program should be accommodated to the local resolution needs of each analyte.
As a tool to elucidate biomolecular structure, ion mobility-mass spectrometry methods are unique in that direct molecular structural information is obtained for all resolved species, largely irrespective of the complexity of the sample.
Depending on the complexity of the sample preparation, there can be variability introduced by different analysts, and in some cases, the integrity of the sample can be compromised during handling.
This prompted a more general concern that in a natural isolate, where the parasite population is heterogeneous, the nested PCR with mutation-specific primers technique can produce erroneous results that underestimate the complexity of the sample.
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Because of the complexity of the samples, they were not able to reconstruct the entire genomes of each of the gut bacteria, just the individual genes.
Due to the complexity of the sampling and despite the many samples that were taken/studied, only three samples will be discussed here.
In other words, the complexity of the sampling algorithm grows linearly in the proposed method, while the traditional SIS grows exponentially.
Elvira et al. also examined a discrete weighting scheme [24] where only a subset of available sampling strategies is selected with the aim of reducing the computational complexity of the sampling process.
However, they do capture the complexity of the samples, as well as the rapid temporal shifts in the evolutionary landscape as a consequence of selective pressure.
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