Exact(32)
We also observed that using the low complexity filtering option [31] did not alter significantly our results.
Each FASTA hit was recalculated without low complexity filtering using the Smith-Waterman algorithm and final Smith-Waterman Scores ≥80 were stored in the database.
To define sequence origins, the assembled sequences were queried (BLASTN, e-value cutoff 1 e-5, no low complexity filtering) [71] against versions 6, 8.3 and trace files of the Hpa genome [25], version 8 of the TAIR Arabidopsis genome [72], version 1 of the Phytophthora infestans [24], P. ramorum and P. sojae [23] genomes and NCBI non-redundant (nr) nucleotide database [73].
The default word-match size between the query and the database sequences was seven with a low complexity filtering ability.
SeqClean also removes polyA repeats and applies low complexity filtering (in addition to performing sequence alignment) to identify similar vector segments in the target EST.
We used TBLASTN (low complexity filtering activated, E < 10−) to identify the most likely position of the missing gene model in the genome.
Similar(28)
Although this may introduce some false positives, we noticed that some meaningful hits were only found without low-complexity filtering.
Reciprocal BlastP (Altschul et al. 1997) searches (e value threshold 1 × 10−6, effective database size 2 × 10, no low-complexity filtering or composition-based statistics) were performed for C. elegans– C. briggsae and D. melanogaster– D. pseudoobscura genome pairs.
Potential IS elements were identified using the BLASTN program (no low-complexity filtering), by querying genome sequences using the ISFinder tool [ 70] in the IS database and querying flanking regions of the draft genome contigs against the NCBI 'nt' database.
The specific program-query-database combinations used for each search are shown in Table 3. BLAST programs were run using a series of E-value thresholds, from E-value ≤ 1 to E-value ≤ 0.00001, with default parameters and without low-complexity filtering.
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