We previously reported the structures of human ETB receptor in the ligand-free form and in complex with the endogenous agonist ET-1, which revealed the activation process of the receptor by ET-1 (ref. 27).
G-2 cells exhibit stable, though heterogeneous expression of SV40-LT, which in complex with the endogenous wild-type p53 accumulated in the nuclei of the majority of cells (Figure 2D), thereby reflecting SV40-LT expression in vivo.
However, effective rerouting relies on incorporation of the FKBP-tagged protein into complexes with the endogenous protein.
The results of the Western blot analysis with anti-Nme1 or anti-Nme1 and anti-FLAG antibody reveal that FLAG/NmeGp1Sd forms complexes with the endogenous Nme1 (lower band in Figure 6C and 6D).
Since both erbB2 and erbB3 were highly expressed by our mammary tumor cell lines and HRG-promoted tumor cell proliferation, we sought physical evidence that the wt-rat neu/ErbB2 could form a complex with the endogenous mouse erbB3.
It could be possibly due to protein complexes associated with the endogenous microRNAs, which are in general lacking for the delivered synthetic therapeutic miRNAs.
In the context of the ES cell experiments, it is not clear why complex formation with the endogenous NKX2-5 protein does not permit more nuclear translocation (in the first paragraph of the subsection headed "DamID identification of NKX2-5 Wtargetstargets
Then, Liu et al. identified calcineurin as the common target of both compounds if and only if they are complexed with the respective endogenous partners, the immunophilins.
The reduced affinity of exon10 binding to full-length protein compared to SH3 domain is consistent with a competition of free exon10 with the endogenous exon10 SH3 complex and thus supports an autoinhibition model involving exon10 SH3 binding.
MFE.htmζ incorporates the human leucocyte antigen (HLA -A*02 transmembrane domain fused to tHLA -A*02ytransmembraneain; this receptor can only form monomers, andomainnable to interact with the endogenous TCR complex [ 12].
We have also disclosed that P-cadherin functional role is dependent on E-cadherin cellular context, since it interferes with the endogenous cadherin/catenin complex, inducing p120-catenin cytoplasmic delocalization and the consequent associated alterations in the actin cytoskeleton [ 29].
