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His-tagged cytbc1 complexes were retrieved from chromatophores partially solubilised by detergent; RC LH1 PufX complexes tended to co-purify with cytbc1 whereas LH2 complexes became detached, consistent with clusters of cytbc1 complexes close to RC LH1 PufX arrays, but not with a fixed, stoichiometric cytbc1 RC LH1 PufX supercomplex.
Yeast protein complexes were retrieved from CYC2008, an updated archive of Curated Yeast Complexes [7].
The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
However, separated complexes were retrieved as well.
From three patients, 57 cumulus oocyte complexes were retrieved.
Immune complexes were retrieved using anti-Rabbit Ig IP Dynabeads.
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Initial coordinates of the Gli1ZF/DNA complex were retrieved from the Protein Data Bank, under the PDB accession code 2GLI (X-ray crystal structure at 2.6 Å resolution) (Pavletich & Pabo, 1993).
The coordinates of the ConI-lactose complex were retrieved from the Protein Data Bank (PDB code 1c1l), and the dimer structure was constructed on the basis of the biological unit matrix.
Next, IRAK-4 tetramer, IRAK-2 tetramers and IRAK-2/IRAK-4/MyD88 IRAK-2/IRAK-4/MyD88 IRAK-2/IRAK-4/MyD88lographicomplex 3MOP, were all hydretrievedms added according to the protonation state at pH=7, which means that all acidic residues (Asp and Glu) are deprotonated and all basic residues (Lys and Arg) are protonated.
A higher mean number of cumulus oocyte-complexes was retrieved in the cumulus oocyte-complexesup (Table II).
Cumulus-oocyte complexes (COCs) were retrieved from ovaries of slaughtered cows by aspiration of follicular fluid of ovarian follicles.
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