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First, 99 random protein complexes are collected as random control protein complexes.
The human protein complexes are collected from the database of CORUM [ 38] and PCDq [ 39].
After mapping on 5,080 diseases and 9,998 proteins, a total of 539 disease-related protein complexes are collected from the CORUM database (released in February 2013) [ 41].
When the sample has been assembled the complexes are collected, either by filtration on a porous polyethylene membrane [ 19] or by centrifugation on an ATR substrate [ 18], and are then readily mounted in the FTIR spectrometer.
Data on protein complexes are collected from the study of individual systems, and more recently through high-throughput experiments, such as yeast two-hybrid (Y2H) [ 1, 2] and tandem affinity purification followed by mass spectrometry (TAP/MS) [ 3, 4].
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Finally, the prepared Lipos/CD-PEI complexes were collected and stored at 4 °C until further use.
Quantitative data about the intercalated complexes were collected by chemical analysis and X-ray absorption spectroscopy investigating the near edge region as well as the extended fine structure.
Thirty SH3 domain-peptide complexes were collected from the PDB.
Immune complexes were collected with protein A-Sepharose and eluted.
The immune complexes were collected by centrifugation and washed following the manufacturer's protocol.
Immunoprecipitated complexes were collected, washed and eluted using Dynal Magnetic Particle Concentrator (Invitrogen).
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