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The binding reactions of melagatran (the active metabolite of ximelagatran) to Factor IIa, meizothrombin (mIIa), and the thrombin thrombomodulin complex were added into the model (RXi1 to RXi4; Table 2).
Dermal T cells stimulated for 3.5 h with PMA and ionomycin, were washed with ice cold PBS and resuspended in 100 µL of PBS plus 2% human serum, whereafter the 24 µL of IL-17A capture complex were added.
For experiments shown in Fig. 2B E, ligation was performed in solution with single hexamer oligonucleotides. 1 pmol of hexamers, 4 units of T4 DNA ligase and 2 µl 5× DNA Ligase buffer (In vitrogen - Life Technologies, Breda, NL) and template/annealer complex were added in a total volume of 10 µl and incubated for 45 minutes at 16°C, followed by a 10 minutes denaturation step at 65°C.
Twenty μl of bait-prey complex were added to the HaloLinkTM resin resuspended in 100 μl binding buffer.
The supernatant was discarded and to the beads antibody chromatin complex were added 240 μl of elution buffer (TE buffer with 2% SDS).
An appropriate EspP derivative, SurA and proteoliposomes containing Bam complex were added successively to 20 mM Tris HCl pH 8. Reaction components were mixed after each addition.
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The complex was added to and altered at many periods and, in consequence, lacks a systematic plan.
When the property was sold in 1918, a stable and kennel complex was added, and the house was converted into a hunt club called the Byram River Beagle Club.
The resulting BR BSA or DIA BSA complex was added into an ARS solution for binding site competition studies.
Following procedure A, the previously produced ZP/HP-β-CD solid complex was added to the water phase of primary emulsion.
Then, the transfection reagent/DNA complex was added to the cells.
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