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Gus contains a SOCS-box domain that is predicted to interact with the Elongin BC complex to recruit Cul-5 containing ubiquitin E3 ligases to proteins, such as Vas, with which it interacts.
Release of the paused polymerase is proposed to be a rate-limiting step and is initiated by signals that allow the polymerase-bound mediator complex to recruit "super-elongation complexes" (SECs) containing, among other proteins, eleven-nineteen lysine-rich lELLemiandELL) and positive transcription elongation factor b (P-TEFb).
However, in some cases the TRAMP complex can also promote exosome-mediated RNA degradation independently of polyadenylation activity and may in that case serve as a scaffolding complex to recruit the exosome to the RNA (Houseley et al. 2007; Rougemaille et al. 2007; San Paolo et al. 2009).
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RdDM likely has three major steps: (1) siRNA biogenesis, (2) production of non-coding transcripts as a scaffold and (3) assembly of AGO-siRNA effector complexes to recruit methylation machinery and target genomic loci.
At the mechanistic level, lncRNAs serve as "scaffolds" providing platforms to assemble RNA-protein complexes, "guides" to recruit RNAprotein complexes to target genes, and "decoys" by binding to and sequestering regulatory proteins away from their target DNA sequences [ 1, 2].
In vacuolar transport, the complex seems to recruit the Rab Ypt7p GEF Vps39p which activates Ypt7p.
The MRN complex helps to recruit and activate key DDR signalling kinases, including ATM at the sites of DNA damage.
BRMS1 has also been reported to interact with the mSin3 chromatin remodeling complex and to recruit histone deacetylases to suppress downstream gene expression [ 28].
At many genes, the PcG complex, PRC2, serves to recruit RNF2 containing complexes.
Remarkably, this complex was able to recruit Crm1 similar to the Rio2 Slx9 RanQLGTP complex.
In the VBC complex, ROC1 functions to recruit the E2 enzyme and also promotes internal complex stability [12], [14].
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