Of these, 62 linkages were intra- or inter-linkages mapping to subunits of the TRiC complex (Supplementary Fig. 1a).
This compensatory role in inducing the switch leads to complete displacement of HEXIM upon titration of R52K into a HEXIM 7SK-SL1apical complex (Supplementary Fig. 9).
Moreover, upon contact with the cognate EphB4 receptor, tyrosine phosphorylation of ephrinB2 leads to disengagement of the complex (Supplementary Fig. 4a).
The final stage of stone growth occurs when three separate stone fragments come into contact, locally dissolve at contact points and interlock to create a larger stone complex (Supplementary Fig. 1b,c and Video 1).
While both proteins attained their compact, functional state at 4 °C, the heat-treated, presumably unfolded myosin eluted at a similar elution volume as the UNC-45:myosin complex (Supplementary Fig. 6a).
This evidence supports our claim that in the presence of Desi3a, which is a bona fide SUMO protease, FLS2 was deSUMOylated resulting in impaired dissociation of BIK1 from the immune complex (Supplementary Fig. 17).
A homology model constructed from the P. falciparum POFUT2 sequence has a very similar predicted structure to H. sapiens and C. elegans POFUT2 (Supplementary Fig. 4A), sufficient to allow the GDP-fucose and TSR domain substrates to be docked into the model to reveal an alignment of catalytic residues and substrates that is reminiscent of a Michaelis complex (Supplementary Fig. 4B, C 26, 27.
In contrast, at the surface of endosomes, GFP-ARPC1A, but not GFP-ARPC1B, colocalised with the retromer that recruits the WASH complex (Supplementary information, Fig. S5).15 Consistently, ARPC1B overexpression strongly increases migration persistence, whereas ARPC1A expression decreases it (Fig. 3j; Supplementary information, Movie S3).
Some acquisition of material originated from several compartments, being difficult to track their origin (Supplementary Fig. 1c, d, complex, Supplementary Movies 2 to 4, 7, 23 and 25) and in most viral inclusions a constant flux of small material in and out was observed (Supplementary Fig. 1a, b, arrows, Supplementary Movies 4 and 24).
A gene reporter assay confirmed that MT2A overexpression reduced transcriptional activity through AP1 complex (Supplementary data 1B).
The pixel size at the specimen level was 2.22 for the FLCC and 2.08 for the CCelastase complex (Supplementary Information).
