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Zhang Y, Arnold E, and Ebright RH, 2014, Crystal structure of Thermus thermophilus transcription initiation complex soaked with GE23077, ATP, and CMPcPP, http://www.rcsb.org/pdb/explore/explore.do?structureId=4OIP, Publicly available at RCSB Protein Data Bank.
Zhang Y, Arnold E, and Ebright RH, 2014, Crystal structure of Thermus thermophilus transcription initiation complex soaked with GE23077, http://www.rcsb.org/pdb/explore/explore.do?structureId=4OIN, Publicly available at RCSB Protein Data Bank.
Zhang Y, Arnold E, and Ebright RH, 2014, Crystal structure of Thermus thermophilus RNA polymerase transcription initiation complex soaked with GE23077 and rifamycin SV, http://www.rcsb.org/pdb/explore/explore.do?structureId=4OIR, Publicly available at RCSB Protein Data Bank.
Zhang Y, Arnold E, and Ebright RH, 2014, Crystal structure of Thermus thermophilus transcription initiation complex soaked with GE23077 and rifampicin, http://www.rcsb.org/pdb/explore/explore.do?structureId=4OIQ, Publicly available at RCSB Protein Data Bank.
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For the Pol II complex with dinucleotide RNA, crystals of the binary complex were soaked for 10 min in cryo protectant supplemented with 35 mM 5′-CA-3′ dinucleotide before freezing.
Finally, the CS Fe complex was soaked in NaOH solution (1.25 mol/L) for 12 h, and the magnetite/chitosan composite was achieved.
In preliminary efforts to obtain cocrystals with pyruvate bound in the active site, crystals of the triazole-ThDP−PDC complex were soaked with pyruvate at 10 mM.
All four α4β4 complex structures soaked with substrates and effectors were solved to the full extent of the data resolution using the previously published 3.95-Å resolution structure (Zimanyi et al., 2012) with all nucleotides removed.
To develop a soft-to-hard tissue interface, we made a decellularized dermis/poly methyl methacrylate) (PMMA) complex by soaking the decellularized dermis in methyl methacrylate (MMA) and an initiator, and then polymerizing the MMA.
A mutation of Lys73 to alanine abolishes the acylation reaction, thus allowing us to trap the Michaelis Menten complex by soaking the K73A BlaC mutant crystal with tebipenem.
The whole complex was then soaked at the center of a TIP3P water box with a margin of 10 Å along each dimension.
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