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"It is one of the [technically] most complex probes ever launched".
Complex probes that utilize all three reaction vessels, such as d-[18F]FAC and l-[18F]FMAU required more steps than one-pot syntheses (i.e., 42 steps).
Then, after stripping, microarrays were hybridized with complex probes made from 5 µg of retrotranscribed total RNA.
After loading, data obtained with vector probe and complex probes were both corrected for neighborhood effects and local background as described [23].
Since the hybridization signal depends on the amount of PCR products spotted onto the microarray, the signal obtained with the vector probe was used to normalize the signal obtained with complex probes [24].
cDNA complex probes from controls or CD49e silenced TECs were Cy5-labeled.
Similar(43)
The complex probe is easy to synthesize.
The complex probe was characterized by FT-IR and TG/DTA.
The complex probe method can be used to detect virus infection levels, transgenic copy quantities, single nucleotide polymorphisms, etc.
To perform real-time detection of specific genes, a new complex probe has been designed and synthesized.
Based on fluorescence resonance energy transfer (FRET), this complex probe is composed of a long-fluorescent reporter probe and a short-quenching probe.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com