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The assay is based on the principle of oxidation of the ferrous ion-chelator complex to ferric ion with the oxidants which are present in the sample.
Open image in new window Figure 8 Mechanism of oxidation of organic compounds with Fe 3+ and H 2 O 2. The inorganic ions inhibit Fenton and photo-Fenton oxidations of organic compounds; their reaction on iron makes complexes of ferrous and ferric ions less reactive for the Fenton reaction, and they can decrease the overall efficiency.
The oxidant molecules within a sample oxidize the ferrous ion-o-dianisidine complex to ferric ion.
Antioxidant capacity of compounds was determined by the reducing potential of an antioxidant reacting with ferric tripyridyltriazine (Fe3+-TPTZ) complex and to produce a blue coloured complex of ferrous tripyridyltriazine (Fe2+-TPTZ) [ 31].
The major cause of solvent effect on the electrode reaction could be attributed to the labile nature of ferric complex of 1,10-phenanthroline.
Oxalic acids behave as photo-active complexes in the presence of ferric ions at acidic pH, which undergo photo-decarboxylation reaction (Equation 5) [18] 18]: Fe III RCO 2 2 + + hυ → Fe 2 + + CO 2 + R · Open image in new window (5).
Sulphate ions can also complex ferrous and ferric ions, which are responsible for the production of HO· radicals and the regeneration of Fe2+ ions.
Utilization of ferric chelate complex of trans-1,2-cyclohexanediaminetetraacetic acid (CDTA) for the oxidative scrubbing of H2S and CH3SH in Kraft mill streams is beneficial from the standpoints of iron protection against precipitation and oxygen-mediated regenerative oxidation of the ferrous chelate CDTA.
FRAP assay, based on the reduction of ferric tripyridyltriazine complex to its ferrous colored form.
In reducing power assay, the reduction of ferric cyanide complex to the ferrous form by donating an electron indicates the presence of reductants in the testing samples.
The assay depends on reduction of ferric tripyridyltriazine complex (Fe (III -TPTZ) to ferrous trIII -TPTZriazine (Fe (II)-toTZ) by a reductant at low pH.
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