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Since biologically relevant substrates might differ from theoretical activities inferred from in vitro experiments – ' just because it can, does not mean it does' – the best method of substrate discovery is to identify protease-cleaved substrates in complex milieus (López-Otin and Overall, 2002; Tam et al, 2004).
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Within this complex milieu, a number of credos uneasily co-exist.
Nevertheless, fibrin presents a complex milieu of signals to embedded cells, many of which are not well understood.
Unfortunately, because the pathlength of light varies due to reflection and interference in a complex milieu of different tissues, absolute concentrations are difficult to estimate.
Gastrointestinal fluid is a complex milieu and it is recognised that gut drug solubility is different to that observed in simple aqueous buffers.
One example is the external region of a cell membrane, known as the glycocalyx, which is dominated by a complex milieu of glycosylated molecules.
It requires working at and across the boundaries between knowledge types — a complex milieu of different value systems, norms, and mental models — and multiple stakeholder-engagement processes which facilitate knowledge exchange and co-production.
The microenvironment of the neuron becomes a complex milieu in which high levels of glutamate provide a source of chronic excitatory neurotoxicity, and the contributions of activated microglial cells lead to further cascades of motor neuron death, perhaps serving to propagate the disease once established.
Although, it seems the more subtle and complex milieu of hollering will be, in the long run, much more difficult to defeat.
After speaking to her at great length, I realized that her insider perspective would be most valuable toward dissecting the complex milieu surrounding the AHA.
In this way, worker maturation occurs in a complex milieu of pheromonal compounds.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com