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These biomimetic motile systems are ultimately able to control their motion, directionality, speed and behaviour in response to clues from their complex biological environment.
Development of rapid, highly selective and sensitive miRNA detection in a complex biological environment has attracted considerable attention.
Finally, the SERS mapping results confirmed that nanoprobe exhibited strong SERS signal intensity, excellent stability in complex biological environment and low toxicity inside live cancer cells.
One of the reasons for our poor understanding is the lack of sensitive and specific analytical methods for real-time measurements of H2S in a complex biological environment.
These limitations include cumbersome preparation of antibody-signal DNA conjugates, complicated and time-consuming protocol, risk of cross-contamination among different samples, no quantification capacity of rolling circle amplification (RCA) reaction, complex primer design, and no tolerance to complex biological environment [33].
These data indicated that in vitro selection with pure biochemical entities (e.g., recombinant protein) in solution may not readily translate into effective ligand binding in a complex biological environment, particularly if the target protein is significantly post-translationally modified.
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Complex biological environments, such as the cell cytoplasm or the mucus lining the airways of the lungs, can pose significant barriers to efficient therapeutic drug and gene delivery.
Recently, the transport of synthetic drug and gene carriers has been investigated quantitatively with real-time particle tracking technology, providing new insight into particle behavior in complex biological environments that is guiding rational improvements in particle design.
In the last few years, SERS became very popular in life science applications due to a great amount of information extracted from complex biological environments such as tissues, cell cultures, and biological fluids [1 3].
In summary, cellPACK unites existing and novel packing algorithms to generate, visualize and analyze comprehensive 3D models of complex biological environments that integrate data from multiple experimental sources.
Here we focused on understanding the role of complex biological environments on VEGF release from hydrogels functionalized with specific VEGF-binding peptides (VBPs).
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