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Fractions were individually dried to completion under vacuum.
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The eluates were collected and dried down completely under vacuum.
Samples were then completely dried under vacuum.
After completion, ethanol was removed under vacuum.
The reaction was monitored using ESI-MS and evaporated under vacuum upon completion.
Eventually the complete phantoms were cooled under vacuum conditions.
After completion of the reaction, the solvent was evaporated under vacuum, and the product was extracted with dichloromethane (CH2Cl2).
Upon confirming the completion of cyclization by ESI-MS, the solvent was evaporated under vacuum and deprotection was performed without purification using a mixture of TFA, H2O, and TES (95:2.5 2.5).
After completion of the reaction (as monitored by TLC), the reaction mixture was evaporated under vacuum to produce a white solid.
After the completion of the reaction, the mixture was extracted with petroleum ether by pH adjustment and dried under vacuum.
After completion of the reaction it was cooled to room temperature and water (5 mL) was added, solid separated was filtered washed with water (5 mL) and dried under vacuum to get the desired xanthandione derivative.
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