The incubation period (91 d) was presumed to far exceed that required to reach a pseudo-steady state to drive reactions closer to completion than the standard residence time (42-h and 18-h in the inert media and limestone stages of the columns, respectively).
All the string discrimination tasks were given after the completion of the standard and visually-restricted string-pulling problems.
Their EC ELISPOT remained positive for the entire course of therapy, although they were declared cured by completion of the standard treatment regimen.
One µl of Ambion DNase I (2 U/µl) was then added and incubated at RT for 15 min. Following completion of the standard protocol, concentration and purity of RNA yield was established by spectrophotometry (NanoDrop, NanoDrop Technologies, Wilmington, DE, USA) and quality confirmed by capillary electrophoresis (Agilent Bioanalyzer 2100 NanoChip).
The minimum required version included data collected as a basic requirement for more limited research studies in settings where completion of the standard instrument is logistically impossible.
Child and parent interviews were conducted simultaneously in separate rooms, shortly after intake and before completion of the standard diagnostic work-up (routine care).
Immediately following the completion of the standard admission EQ-5D, patients were then given a blank EQ-5D questionnaire and a brief scripted statement was read by the clinician conducting the assessment.
If a standard treatment exists, it is necessary to determine the optimal timing of cancer peptide vaccine introduction: prior to, during or after the completion of the standard treatment and, in the case of treatment during the same period, as monotherapy or combination therapy.
The expected duration of adjuvant therapy was calculated from the number of days elapsed since diagnosis or the first prescription, if earlier, until either recurrence or death, end of the sample period, or the completion of the standard 5-year therapy.
