The BAC DNA (50 ng) was digested to completion with DraI, separated on 1% agarose gels and transferred by capillary action onto Hybond-N+ membranes (Amersham Pharmacia Biotech, Arlington Heights, IL, USA) using standard techniques.
Because up to 5 months separated completion of the baseline survey and workshop attendance, intervention subjects repeated the baseline survey directly preceding the workshop to assess whether baseline results changed (e.g. due to secular trend or in response to being selected to attend the workshop).
The progress of the reaction was monitored by TLC; after completion, the product was separated by filtration, and the residue so obtained was used for the regeneration of reagent 1 by the addition of molecular bromine in a controlled manner.
After transcription completion, the RNA was separated from the reaction through binding of the dual SRP tag to its protein partner (a domain of the T. maritima Ffh protein (TmaM)) immobilized on a resin, and then recovered by elution in the presence of imidazole (Figure 1A).
Finally, the presentation of each condition was separated by the completion of one of three tasks: the basic arithmetic skill test, the Mathematics Anxiety Scale, or the attention switching task; the order of these three tasks was counterbalanced across participants.
After completion of the reaction, nanospheres were separated using an external magnet and washed with ethanol and water, dried and reused in another set of reaction.
To recover the catalyst, after completion of the reaction, catalyst was separated and washed with ethanol and then dries the solid residue.
The completion of the Embarcadero Freeway in 1957 separated the old building from the fast-growing downtown area and obscured all but the pinnacle of the tower from the rest of the city.
After completion of each step, the solvent was separated and fresh reactants were added without adding fresh DABCO to the medium.
After completion of the reaction, the catalyst was separated by an external magnet, washed with CH2Cl2, dried, and reused for a consecutive run under the same reaction conditions.
