On completion, samples were snap-frozen in liquid nitrogen.
On completion, samples were returned to the laboratory and stored at –20°C.
After study completion, samples were defrosted at 4°C overnight and vortexed; equal volumes of the two samples from each phase of the study were then combined and aliquoted.
Self-completion (sample of 19): average response rate of 25%%, standard deviation of 16.5 %.
The response rate varied according to surveying method: Self-completion (sample of 19): average response rate of 25%%, standard deviation of 16.5 %.
Times required for the completion of sample cutting ranged from approximately 1 min (sample A) to approximately 2.7 min (sample C).
The sampling strategy was convenient sampling where consecutive children were enrolled till completion of sample size.
The test phase began 90 min after completion of sample Phase 2. The pairs of objects were matched so that the object from Trial 1 of sample Phase 1 was paired with the object from Trial 1 of sample Phase 2, and so on.
Sample sizes from each site were unknown until the completion of sampling and logistical constraints prevented revisiting sample sites.
We note that further control experiment of air exposure by completion of sample preparation in the globe box is highly demanded as a future study.
After 30 sessions of DNMTP training, the duration of the back lever presentation following the completion of sample lever responses was also gradually lengthened by imposing a set of delays of 1, 3, 5, 7, or 9 s (sessions 31 60), 1, 5, 7, 9, or 13 s (sessions 61 80) and finally extended to 1, 5, 10, 15, 20 s (sessions 81 100).
