By its completion, all rats would run from one side of the maze to the other and displace an object covering a food well to reach food rewards.
This approach is enhanced by the completion of the rat genome sequence by the Rat Genome Sequencing Project Consortium and the rapidly growing databases of rat polymorphisms, including those of The Rat SNP Consortium.
After the completion of testing, rats used for behavioral testing were deeply anesthetized with sodium pentobarbital and their brains were removed and fixed in 10% paraformaldehyde overnight and then cryoprotected in 20% sucrose for at least 2 d.
On the day following completion of habituation, rats were placed individually in an arena for 30 min and their activity recorded.
Following the completion of testing, rats were deeply anesthetized with sodium pentobarbital, microinjected either with CRF (500 ng) or ink and 1-hr later perfused transcardially with buffered saline, followed by a buffered 4% paraformaldehyde solution.
At the completion of testing, rats were given an overdose of pentobarbital solution (Euthatal, Merial Animal Health, Harlow, United Kingdom) and transcardially perfused with saline, followed by a 4% formalin solution.
Since the completion of the rat reference genome in 2003, whole-genome sequencing data from more than 40 rat strains have become available.
Upon completion of catheterization, the rats were connected to a monitor to observe blood pressure and heart rate throughout the procedures.
Upon completion of the study, rats were euthanized and the hippocampus was dissected out and collected for transcriptome analyses.
At the completion of the experiments, rats were euthanized with an overdose of pentobarbital (100 mg/kg) and transcardially perfused with cold saline followed by cold 4% paraformaldehyde.
