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After completion of the recordings, Lucifer yellow was injected into the recorded cell by applying a hyperpolarizing current.
On completion of the recordings, mice were deeply anesthetized with chloral hydrate and perfused transcardially with saline (50 ml) followed by 10% formalin in phosphate buffer saline (PBS) (200 ml).
Following completion of the recordings, coverslips were fixed with 4% paraformaldehyde for 15 min at room temperature and further immunostained for either βIII-tubulin (Sigma, T8660, 1 1000) or Neurofilament (Sigma, N2912, 1 1000).
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After completion of the baseline recordings in 20 experiments, d-sotalol was added to the buffer solution: d-sotalol was applied at a concentration of 10−4 mol/L, and the stimulation protocol was repeated again.
On November 5 , 2012Ted Tsung, the North American producer for Tales of Xillia, announced the completion of the English voice recordings.
Ultimately, the completion of the album included recordings by Alan Jackson, Norah Jones, Jack White, Lucinda Williams, Vince Gill, Rodney Crowell, Patty Loveless, Levon Helm, Jakob Dylan, Sheryl Crow and Merle Haggard.
After completion of the 1 3 ratio recordings, counterpulsation was returned to a 1 1 ratio and a further six minute recording collected.
After completion of physiological recordings or tracing experiments the lesion site was reconstructed from cross-sections and measured as a percentage of the area of the intact cord (Fig. 1B).
Following completion of the interviews, transcripts created from the recordings and interviewer notes were systematically coded for thematic content independently by two reviewers.
Glial fibrillary acidic protein (GFAP) immunostaining was also routinely performed, as previously described, and examined at bregma 0 and –6.5 mm in most rpFPI and sham-injured animals after the completion of recordings.
Upon completion of ERG recordings, fish were dark-adapted for 1 hour and then killed by cervical transection.
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