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The outcome of the histological test of each patient was released at the end of the study, following completion of the FISH analyses.
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After completion of the study, fish were retained as stock for unrelated research.
On completion of the trials, all fish were screened under anaesthetic for any parasite transmission and accurate worm counts.
Upon completion of the temperature trial, fish were sacrificed using AQUI-S® solution [ 36] and dissected immediately to remove their livers.
Upon completion of the FCA, individual fish correspondence scores were used to calculate an overall mean, across all populations, across each of the three FCA axes (see below) using the general spreadsheet-based statistical software program PAST version 1.98 (Hammer et al. 2001).
On completion of the behavior studies, the fish were euthanized and sex was determined by gonadal morphology.
The river was dammed in 1961, leading to fish having no access to upstream spawning areas in the period after dam construction and the completion of a fish ladder.
Upon completion of the experiment at 96 hours, the fish were snapped frozen in liquid nitrogen and stored at -80°C for subsequent analysis.
Recent completion of the DNA sequencing for several fish species has spurred the development of DNA microarrays allowing investigators access to toxicogenomic approaches.
They say it is likely to take three years from the completion of dredging for fish to re-establish their breeding grounds.
On completion of the first flume trials, six focal fish from the flow action trials and eight focal fish from the static condition were infected with G. turnbulli following standard procedures (e.g. Richards et al. 2010).
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