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After completion of the extraction, the methanol solvents were evaporated using rotatory evaporator.
After the completion of the extraction, the sample vial is inserted into an ice bath to freeze the organic phase.
The material remaining upon completion of the extraction procedure was termed the "residual" component, consistent with established protocols.
After completion of the extraction, the liquid contents of the reaction flask were poured into a separatory funnel, and an aliquot of the bottom perchloroethylene layer was drawn off for analysis by HPLC.
At this point, samples were stored at −20°C prior to completion of the extraction and QT-NASBA analysis.
At this point, samples were stored at −20°C and transferred to the laboratory for completion of the extraction and QT-NASBA analysis.
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BDC contributed to the search strategy, screening of studies, completion of the data extraction forms and the quality assessment tools and finalised the draft of the paper.
After completion of the DNA extraction, 2 μl of the sample was separated by gel electrophoresis for visualization of genomic DNA.
On completion of the data extraction, a feasibility assessment was conducted to assess a priori which studies were sufficiently homogenous to be combined in a robust meta-analysis.
VV contributed to the development of the research protocol, screening of studies, completion of the data extraction forms and the quality assessment tools, and wrote the first draft of the paper.
After completion of the salt extraction, mitochondrial lysate was ultracentrifuged and fractions enriched in mitochondrial membranes as well as a supernatant enriched in soluble and mebrane-stripped proteins were used for further analyses.
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