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Upon completion of the addition, an additional 0.25 mL of 5%% hexachloroplatinic acid solution was added and the mixture was stirred at 100 °C for 45 min. GC analysis of the mixture indicated the presence of some unreacted pentachlorodisilapropane.
Upon completion of the addition, the mixture was stirred at 25 °C for 15 h.
Upon completion of the addition, the temperature of the reaction mixture was allowed to warm to room temperature.
After completion of the addition, the reaction mixture was warmed up to room temperature for another 2 h, and was cooled again with ice bath.
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Upon completion of the silane addition, an additional 0.25 mL of the catalyst solution was added and the reaction mixture heated at 95° for 45 min. Distillation provided 80 g (70%%) of the title organosilane.
After completion of the metal addition, the mixture was stirred for an additional 30 min.
After completion of the olefin addition, the reaction was heated for an additional 3 h at 130 °C.
Stirring at 150 rpm was continued for another 2 h at 30°C upon the completion of the TBT solution addition.
Our hypothesis was that completion of the bundle with addition of lactate clearance will further decrease mortality compared with completion of the bundle without lactate clearance.
Harders et al. successfully reduced their turnover time by 37% by supplying pagers for all theatre assistants which were triggered 5 minutes prior to completion of the case in addition to standardisation of equipment.
Using both a coupled enzyme assay and intrinsic RNAP fluorescence, the Johnson laboratory has reported that the incoming NTP-Mg2+ is necessary for rapid rates of PPi release, indicating that the NTP-Mg2+ acts as an allosteric effector for completion of the previous bond addition step.
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