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Following completion of synthesis, the crude peptide was cleaved from the resin and fully deprotected with Reagent B [26], [27].
The 3 µm diameter protocells have approximately 104 copies of the receptor protein on each particle after the completion of synthesis.
After the final cycle, the temperature was maintained at 72°C for 7 minutes to allow completion of synthesis of amplified products.
Next, seven additional enzymes are required for the completion of synthesis of the minimal LPS structure Kdo2-lipid A, which acts as a substrate for further sequential incorporation of various sugars by specific glycosyltransferases.
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Detailed knowledge of the mechanism of primer synthesis is lacking, but it must involves initiation, extension and completion of RNA synthesis by primase, intramolecular hand-off of the RNA oligonucleotide to Pol α and limited RNA extension with deoxynucleotides (dNTPs) (Brooks and Dumas, 1989; Kuchta et al., 1990; Copeland and Wang, 1993; Sheaff and Kuchta, 1993; Sheaff et al., 1994).
FTIR and 1H NMRspectroscopy characterization confirmed completion of polyurethanes synthesis.
The completion of phospholipid synthesis occurs in the ER.
In eukaryotes, for example, mitosis is dependent on the completion of DNA synthesis.
Nanoparticle formation was initiated within 5 min. The completion of nanoparticle synthesis was achieved after 1 h as identified in Figure 4.
An inactive APC/C stabilizes Geminin, Cyclin A and Cyclin B1, thereby securing completion of DNA synthesis and progression through G2-phase.
Some dependencies can be relieved by mutation (mitosis may then occur before completion of DNA synthesis), suggesting that the dependency is due to a control mechanism and not an intrinsic feature of the events themselves.
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