All samples were analysed within 2 weeks of sample collection.
Five months after completion of the sample collection, and in order to evaluate intraobserver agreement, a second measurement process was performed both qualitatively and quantitatively using an anthropometer.
After completion of the blood sample collection, DNA extraction was started from white blood cells.
The sampling strategy was convenient sampling where consecutive children were enrolled till completion of sample size.
Thus, any confounding effect of different sample collection times, particularly after EEN initiation and completion, was naturally accounted for within the analysis.
After completion of clinical ocular examinations and sample collection, treatment was offered to study and control villages.
The nasal sampling procedure for the screening of S. aureus nasal carriage was standardized to ensure accurate sample collection and completion of microbiological procedures.
Upon completion of data collection, a random sample of 10% of the questionnaires was checked for completeness and accuracy to guarantee the quality and integrity of the collected data.
A financial compensation was given for every blood sample collection at completion of the study.
Follow up for 10 years following completion of the collection of DL samples with regards breast cancer development is planned.
