On completion of the recordings, mice were deeply anesthetized with chloral hydrate and perfused transcardially with saline (50 ml) followed by 10% formalin in phosphate buffer saline (PBS) (200 ml).
Upon completion of ERG recordings, fish were dark-adapted for 1 hour and then killed by cervical transection.
After completion of physiological recordings or tracing experiments the lesion site was reconstructed from cross-sections and measured as a percentage of the area of the intact cord (Fig. 1B).
Following completion of the recordings, coverslips were fixed with 4% paraformaldehyde for 15 min at room temperature and further immunostained for either βIII-tubulin (Sigma, T8660, 1 1000) or Neurofilament (Sigma, N2912, 1 1000).
After completion of the baseline recordings in 20 experiments, d-sotalol was added to the buffer solution: d-sotalol was applied at a concentration of 10−4 mol/L, and the stimulation protocol was repeated again.
The response rates in completion of questionnaires and diary recordings were also acceptable.
On November 5 , 2012Ted Tsung, the North American producer for Tales of Xillia, announced the completion of the English voice recordings.
Ultimately, the completion of the album included recordings by Alan Jackson, Norah Jones, Jack White, Lucinda Williams, Vince Gill, Rodney Crowell, Patty Loveless, Levon Helm, Jakob Dylan, Sheryl Crow and Merle Haggard.
The circadian phase of the suprachiasmatic nucleus was inferred from the melatonin circadian rhythm extracted from salivary samples collected after completion of each hourly pupil recordings.
