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We observed substantial variation in native germ cell numbers between different mice, especially in early development; this natural variation likely reflects a combination of differences in the timing of the completion of migration, proliferation of initial germ cell populations, and arrest or entry into meiosis, as well as genetic background effects as previously reported [2], [41] [43].
The entire project from requirements gathering through completion of migration of the system took three years.
At stage 13, following completion of migration, the PMECs form a columnar epithelium with apico-basal polarisation of proteins, such as Filamin-1 (Fig. 6A).
Using 3-D quantitation of dendritic branching patterns, based on a synchrotron X-ray microscopy with Golgi staining, we show that completion of migration is essential for the development of planarity in PC dendrites.
(A – F ) MIP of Z-stacks of pLLP stained with ZO-1 (blue) and GFP (green) antibodies and phalloidin (red) in control (A, C, E ) and amotl2a morphant (B, D, F ) cldnb gfp embryos at 30 hpf. (G, H ) MIP of overview images of control (G ) and amotl2a morphant (H ) embryos after completion of migration.
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These results indicate that the planarity of PC dendrites requires completion of cell migration but it is independent of the presence of Crk/CrkL.
In contrast, metastatic cancer cells often have weak cell-matrix adhesion, so strengthening their attachment to the matrix facilitates the completion of cell migration cycles.
The maternal and paternal pronuclei were not close to each other, indicating that the embryo's development arrested before completion of pronuclei migration.
As neurons continue to migrate well into postnatal stages, we addressed the final positions of neurons generated from all Tbr2+ IPCs in Ai9 brains at postnatal Day 21 (P21, N = 4 animals) after the completion of neuronal migration (Fig. 2 A).
This point in gestation marks the completion of the migration of germ cells to the gonadal ridge in mice and other studies with these mice have shown that the tumours are initiated in this critical time period.
Some of the abnormally accumulated cells in the Nf1hGFAPCKO ML were found on the pial surface of the cerebellum, corresponding to the EGL a structure that transiently exists during development, but disappears after the completion of GC migration (arrows, Figure 1Ab′′).
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