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Corresponding white light images were acquired before the administration of hypericin and/or after the completion of fluorescence imaging to avoid photobleaching of hypericin because of white light illumination.
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All samples were processed for conventional histo-pathological examination after completion of the fluorescence imaging studies.
Histochemical staining for GUS activity was performed as described previously [ 38] following completion of GFP fluorescence detection.
Following completion of a run, fluorescence readings are exported into an Excel workbook.
Following the completion of PCR reactions, fluorescence was determined on a Tecan 384 Ultra reader (Tecan USA, Durham, NC).
In quadruple label assays Eu and Sm are measured in the BFPP-based enhancement solution subsequent to the completion of the biospecific reaction and the fluorescence of the dissociated Tb and Dy are detected after addition of a dipicolinic acid derivative containing solution that raises the pH to neutral.
The observed pattern of fluorescence responses under conditions of Asc insufficiency for the completion of Cr VI) reduction parallels the appearance of Cr V) in ESR studies.
Upon completion of PCR, the DNA samples are subject to a temperature gradient, and the loss of fluorescence signal resulting from denaturation gives each DNA sample a unique melting curve that is detected by the real-time PCR system [ 19].
After the completion of exposure time specific for each photosensitizer (for alasens-induced PPIX and for radachlorine 3 h), a session of fluorescence diagnostics with evaluation of area of tumor and planning of laser irradiation fields was performed.
Drug treatment commenced 6 h after the completion of labeling and progressive dilution of CFSE fluorescence indicates cell proliferation.
At the completion of the assay, cells were examined by fluorescence and phase-contrast microscopy to confirm that fluorescent areas corresponded to attached bacteria.
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