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The integrity of the endoplasmic reticulum was confirmed by retention of SpARC1 in pellet fractions upon completion of enzyme assays and the ability of the homogenates to actively accumulate 45Ca in a thapsigargin-sensitive manner.
For example, the study of enzyme kinetics requires completion of enzyme assays which measure, for instance, the change in substrate or product concentration over time in order to calculate a reaction rate.
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After the completion of enzymatic reaction, the enzyme was inactivated by heating at 100°C.
The statistics of steps and dwell times in reversible molecular motors differ from those of cycle completion in enzyme kinetics.
Following the completion of splicing, the lariat-debranching enzyme resolves the branch point to generate a pre-miRNA-like hairpin that can be exported from the nucleus and further processed by Dicer.
This could be due to the reduction in enzyme activity rather than completion of reaction, since it was again increased after re-adding enzyme after 5 h of proteolysis.
TruD and ThcD were essentially identical in apparent reactivity, whereas our PatD enzyme preparation was relatively less efficient, such that a higher amount of enzyme was required to allow reaction completion at the same time-point.
Treatment was carried out for 150 days and after the completion of treatment, serum and liver enzymes and other biochemical parameters in the serum and liver were assessed.
Patients were instructed to stop taking their usual pancreatic enzyme replacement therapy before the start of the run-in period (Visit 1); they were permitted to take their normal dose of pancreatic enzymes following completion of the first stool collection up to randomisation (Day 1/Visit 2).
Five samples were taken of each formula for enzyme determination upon completion of substrate colonization.
In a previous curation effort, the automatic processing within Argo was found to be adequate for the completion of ∼84% of a drug and enzyme annotation task, the remainder of which was completed manually by a domain expert using Argo's manual annotation editor (8).
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