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We collected samples from all 47 locations within an 8-hr period on each sample day and delivered them to the Chemistry Centre of Western Australia for analysis at the completion of each sampling run.
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Following completion of each study, the samples were dried in a 60 °C oven for 7 days until a constant dry weight was achieved.
Sample sizes from each site were unknown until the completion of sampling and logistical constraints prevented revisiting sample sites.
In the framework of a reproducibility study, aspects of the analytical platform such as the delivery of a sample to the analytical laboratory and the completion of sample analysis (e.g., sample processing), chromatographic separation and electrochemical detection, and peak identification and quantitation were considered.
A maintenance infusion was administered with 8 mL/kg/h Ringer's acetate solution from the start of anesthesia to completion of blood sampling.
All subjects were given the special tubes and phlebotomy supplies to submit various blood and breast milk samples and contacted on a regular basis for the completion of the sampling.
On day 7, after the completion of the sampling for the 48 h post-dose plasma apremilast concentration determination, oral doses of rifampicin 600 mg once daily were administered for 15 days (days 7 21).
Upon completion of each exercise bout a final venous sample (7 mL) was collected while participants remained on the cycle ergometer as previously described.
The circadian phase of the suprachiasmatic nucleus was inferred from the melatonin circadian rhythm extracted from salivary samples collected after completion of each hourly pupil recordings.
Upon completion of sampling, the bag was replaced over the roller and the entire sampling apparatus was removed from the bin.
All samples were stored on wet ice in the dark until completion of sampling and delivery to the lab.
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