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The response rate at baseline was high and there was an acceptable response rate (77.6%) at the 6-year follow up survey with high completion of dataset.
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As part of this nested study we were provided with an extract of baseline questionnaire data for these 39 women by the Born in Bradford study, in advance of completion of the dataset for the main cohort for which recruitment is still ongoing.
Because the quantification method described above was not applicable to the narrow layers 4c and 5, and for mere completion of the dataset, these layers were analyzed using single line OD measurements for each section (length of 1 mm, ImageJ software v1.45s) with the lines placed parallel to the layers in area 17.
Also, we did not include Serratia symbiotica [ 95] because its genome only became available after completion of our datasets.
CEGMA software [ 73] (version 2.4.010312) has been used to assess the completion of the transcript dataset.
The completion of the FC dataset, taking data preparation, annotation of characters and taxa, ontology development, and team discussions into account, required a total of two person-years (4136 h), with a resulting overall annotation rate of 0.65 characters per hour.
The quality assurance system and CAPI method worked well and resulted in quick completion of the analytical datasets.
Upon completion of the two datasets software algorithms were developed to categorize every exon into one of the 29 POEM categories.
When we considered only full-length A. pompejana proteins, the MPI + JGI + Genoscope dataset was 74% complete for animal-specific proteins, compared to the 55% completion of the JGI + Genoscope dataset.
In order to estimate the completion of the A. pompejana datasets, we performed extensive BLASTP searches in the proteome of 15 animal and 8 additional eukaryotic species.
Museo de Historia Natural Noel Kempff Mercado provided the necessary datasets for the completion of this project.
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